New insight to IL-23-IL-17 axis in Iranian infected adult patients with gastritis : effects of genes polymorphisms on expression of cytokines
| Journal | Volume 78 - 2015 |
| Issue | Fasc.2 - Original articles |
| Author(s) | Hedayatollah Shirzad, Nader Bagheri, Fatemeh Azadegan-Dehkordi, Behnam Zamanzad, Esmail Izadpanah, Mohammad Abdi, Ghasem Ramazani, Mohammad Hossein Sanei, Hiresh Ayoubian, Abbas Ahmadi, Sirous Jamalzehi, Parya Aslani, Farid Zandi |
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| (1) Cellular & Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran ; (2) Department of Immunology, School of Public Health, Teheran University of Medical Sciences, Teheran, Iran ; (3) Departments of Physiology and Pharmacology, Kurdistan University of Medical Sciences, Sanandaj, Iran ; (4) Department of Clinical Biochemistry, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran ; (5) B.Sc. of Nursing. Endoscopy Department of Hajar hospital, Shahrekord University of Medical Sciences, Shahrkord, Iran ; (6) Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran ; (7) PhD Scholars at University of Saarland, Saaraland, Germany ; (8) Kurdistan Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran ; (9) Department of immunology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. |
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Background and Objective : Chronic inflammation is the hall- mark of the pathogenesis of H. pylori-induced gastric cancer. IL- 17A and IL-17F are inflammatory cytokines expressed by a novel subset of CD4+Th cells and play critical function in inflammation. We evaluated the relationship between IL-17A G197A, IL-17F A7488G and IL23R+2199 A/C polymorphisms with IL-6, IL-17, IL-21, IL-23 and TGF-ss1 mRNAs expression in regard to H. pylori infection with chronic gastritis. Materials and Methods : Total RNA and genomic DNA were ex- tracted from gastric biopsies of 58 H. pylori-infected patient with gastritis. Afterward, mucosal IL-6, IL-17, IL-21, IL-23 and TGF-ss1 mRNAs expression and polymorphisms in IL-17A G197A, IL-17F A7488G and IL-23R +2199A/Cin gastric biopsies were determined by real-time PCR and PCR-RFLP. Results : Our results show that IL-17A G197A, IL-17F A7488G andIL23R +2199A/C polymorphisms have no effect on mucosal expression of IL-6, IL-17, IL-21 and TGF-ss1 mRNAs expression in H. pylori-infected patients with chronic gastritis. Conclusion : These results suggest that IL-17A G197A, IL-17F A7488G and IL23R +2199A/C polymorphisms no alter mucosal cy- tokine pattern in Iranian patients with H. pylori-associated gastritis diseases. (Acta gastroenterol. belg., 2015, 78, 212-218). |
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© Acta Gastro-Enterologica Belgica. PMID 26151690 |
